scholarly journals ESTIMATING ANIMAL ABUNDANCE USING NONINVASIVE DNA SAMPLING: PROMISE AND PITFALLS

2000 ◽  
Vol 10 (1) ◽  
pp. 283-294 ◽  
Author(s):  
L. Scott Mills ◽  
John J. Citta ◽  
Kevin P. Lair ◽  
Michael K. Schwartz ◽  
David A. Tallmon
2018 ◽  
Vol 20 (2) ◽  
pp. 137-144 ◽  
Author(s):  
Linda A. Hatfield ◽  
Rebecca K. Hoffman ◽  
Rosemary C. Polomano ◽  
Yvette Conley

Purpose: To recruit healthy full- and preterm infants into genetic research and determine the effectiveness of a noninvasive DNA sampling technique for comparing epigenetic modifications. Background: Noxious stimuli during a vulnerable period of infant neuronal plasticity may trigger long-term epigenetic changes affecting neurodevelopment, pain modulation, and reactivity. Recognizing epigenetic pain findings is problematic because parents are reluctant to enroll newborns into genetic research. Methods: Design: Within-subject change over time candidate-gene DNA methylation association study. Setting/ sample: Urban teaching hospital’s neonatal intensive care unit and newborn nursery. Convenience sample of healthy full- (>37 weeks, n = 6) and preterm (<37 weeks, n = 6) infants. Procedure: Parents participated in a genetic presentation prior to informed consent. Infant buccal saliva was collected after admission to the unit and prior to discharge. Analysis: The methylation pattern at the 5′ end of µ-opioid receptor gene ( OPRM1) was examined. DNA was treated with bisulfite to convert all cytosines to uracil residues, leaving methylated cytosines unchanged. Sequencing of untreated and bisulfite-converted DNA was carried out. The sequences of unconverted and bisulfite-converted DNA were aligned with ClustalW, fidelity of the polymerase chain reaction and the sequencing reaction evaluated, and the methylation pattern identified. Results: Recruitment and assessment of a noninvasive DNA sampling technique for comparing epigenetic modifications were successful; however, infant stress did not produce a change in OPRM1 methylation expression. Relevance: This study established the feasibility of recruiting healthy full-term infants into genetic research and the effectiveness of noninvasive DNA sampling for comparing epigenetic modification in infants.


2019 ◽  
Vol 53 (23-24) ◽  
pp. 1439-1449
Author(s):  
Yamna Karssene ◽  
Raquel Godinho ◽  
Mohsen Chammem ◽  
Berardino Cocchiararo ◽  
Said Nouira ◽  
...  

2019 ◽  
Vol 96 ◽  
pp. 118-123 ◽  
Author(s):  
Yamna Karssene ◽  
Carsten Nowak ◽  
Mohsen Chammem ◽  
Berardino Cocchiararo ◽  
Said Nouira

2021 ◽  
Author(s):  
Emma A. Higgins ◽  
Doreen S. Boyd ◽  
Tom W. Brown ◽  
Sarah C. Owen ◽  
Adam C. Algar

2015 ◽  
Vol 39 (2) ◽  
pp. 413-421 ◽  
Author(s):  
Stephanie M. Demay ◽  
Janet L. Rachlow ◽  
Lisette P. Waits ◽  
Penny A. Becker

Genes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 26
Author(s):  
Colin Charles Tièche ◽  
Markus Dubach ◽  
Martin Zieger

In terms of crime scene investigations by means of forensic DNA-analyses, burglaries are the number one mass crime in Switzerland. Around one third of the DNA trace profiles registered in the Swiss DNA database are related to burglaries. However, during the collection of potential DNA traces within someone’s residence after a burglary, it is not known whether the sampled DNA originated from the perpetrator or from an inhabitant of said home. Because of the high incidence of burglaries, crime scene investigators usually do not collect reference samples from all the residents for economical and administrative reasons. Therefore, the presumably high probability that a DNA profile belonging to a person authorized to be at the crime scene ends up being sent to a DNA database for comparison, has to be taken into account. To our knowledge, no investigation has been made to evaluate the percentage of these non-perpetrator profiles straying into DNA databases. To shed light on this question, we collected reference samples from residents who had been victims of recent burglaries in their private homes. By comparing the profiles established from these reference samples with the profiles generated from trace DNA, we can show that the majority of the DNA samples collected in burglary investigations belong to the residents. Despite the limited number of cases included in the study, presumably due to a crime decline caused by the pandemic, we further show that trace DNA collection in the vicinity of the break and entry area, in particular window and door glasses, is most promising for sampling perpetrator instead of inhabitant DNA.


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